No, Doxosome is produced for animal research purposes only. It should only be given to animals during controlled laboratory experiments. Any use of these products in humans or animals for treatment purposes is legally prohibited by U S Food and Drug Administration.
2. What is the proper dosage of a Doxosome injection?
This answer varies due to individual experimental conditions. Our database may provide some helpful suggestions. The database currently contains a small collection of Doxorubicin liposome related scientific studies. More research will soon be added. Additional research on your part may also be required to determine the proper dosage for your experimental stipulations.
3. Can I use any fluorescent tag?
Yes, however, Doxorubicin itself is a fluorescent molecule therefore you must be careful not to use a fluorescent tag that is within the same range (excitation wavelength 480 nm; emission wavelength 580 nm). Most of the regular colored fluorescent tags are not compatable. You need to make sure that your flurecent tag will not have any excitaion or emission that will interfere with Doxorubicin.
4. Is PEGylated Doxosome better than Non-PEGylated Doxosome?
This is not necessarily true. Myocet which is a Non-PEGylated Doxorubicin liposome has shown in clinical trials remarkable tumor shrinkage capability.
5. Why do PEGylated products come in 1 vial kits while Non-PEGylated products come in 3 vial kits?
Non-PEGylated products require the remote loading of the active ingredient, Doxorubicin, using a pH gradient. The pH gradient allows the maximum amount of Doxorubicin to be loaded into the plain Doxosome or Immunodox liposomes. One drawback of this loading technique is the weak stability of the pH gradient. Therefore immediately before drug administration you will use the 3 vials in the kit to create a fresh pH gradient.
In addition, Non-Pegylated liposomes are created with unsaturated phospholipids which form loosely packed liposomes around the encapsulated drug. This means that once the drug is loaded into the liposome it slowly leaks its contests in a matter of a week inside the vial. By using the 3 vial kit and loading the Doxorubicin directly before administration all of the encapsulated drug will be delivered to the test subjects.
The PEGylated products on the other hand use a ammonium sulfate gradient that remains stable over time. They are also formed using saturated phospholipids which form a much more compact liposome around the encapsulated drug. This allows Encapsula to load the drug for you.
6. Will Encapsula conjugate the antibody for us?
Encapsula NanoSciences does provide this service as a custom formulation. However, the kits that are sold through our product catalog contain various types of reactive lipids on the surface of the liposomes that allows different method of conjugation. You can easily follow the few steps based on the protocols on the website and do the conjugations yourself.
7. Which method of conjugation is better?
This depends on your experimental stipulations. Specific applications will work better depending on the several factors such as targeted site or conjugation antibody. In some instances the best way to determine the most effective conjugation method is through trial and error. You may also be able to refer to our database for helpful suggestions.
Copyright by Encapsula NanoSciences 2014
Use this form to find things you need on this site