Conjugation of DSPE-PEG(2000) PDP to thiol of antibody fragment
Free sulfhydryl groups on fragmented antibodies can couple to active disulfide containing lipids to form disulfide bridges. Pyridyl dithiol (PDP) is perhaps the most popular type of thiol-disulfide exchange functional group. It will readily undergo an interchange reaction with a free sulfhydryl to yield a single mixed disulfide product. This is due to the fact that the pyridyl disulfide contains a leaving group that is easily transformed into a non reactive compound not capable of participating in further mixed disulfide formation. PDP reagent is highly efficient in forming disulfide bonds with sulfhydryl containing molecules. In addition, the PDP leaving group has unique spectral properties that allow the measurement of sulfhydryl coupling by monitoring the increase in absorbance at 343 nm.
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